Novozym® 435 and Lipozyme® RM IM as Biocatalysts for Benzyl Benzoate Synthesis

With the ever-increasing demand for clean technology in industrial sector, natural methods, such as enzyme-catalyzed, represent a sustainable alternative to chemical processes. In this context, synthesis of benzyl benzoate ester using commercial immobilized lipases was evaluated. For this, kinetic study carried out determine reaction time (24 h) and enzyme concentration (10 wt%). Then, 22 full factorial design proposed evaluate effect molar ratio (benzyl alcohol benzoic anhydride) temperature on conversion presence tert-butanol solvent. Novozym® 435, maximum (32%) achieved at 60 ºC, 1:5 (alcohol anhydride). A 51% obtained Lipozyme® RM IM 40 ºC 1:5. The showed moderate antimicrobial action against S. aureus (MIC = 0.05 mg μL-1). results, conclusion that methodology experiments adequate system allowed optimization production benzoate.